The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Millipore Detect Nitrotyrosine Using This Anti-Nitrotyrosine Antibody, Clone 2a8.2 Validated For Use In Ih(P) & Wb. - Mill (Additional S&H or Hazmat Fees May Apply)
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Detect Nitrotyrosine using this Anti-Nitrotyrosine Antibody, clone 2A8.2 validated for use in IH(P) & WB.
Key Applications
Immunohistochemistry (Paraffin)
Western Blotting
Application Notes
Immunohistochemistry: 1:50-1:100 on paraffin embedded of a previous lot, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the MAB5404 was for 10 minutes and the reaction was developed with the Chemicon IHC-Select, HRP-DAB kit (Catalog number DAB150).
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Nitrated KLH.
Clone
2A8.2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Reacts with nitrotyrosine.
Isotype
IgG2b
Species Reactivity
All
Antibody Type
Monoclonal Antibody
Purification Method
Protein A Purfied
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on Nitrotyrosine Immunoblot Controls.
Western Blot Analysis: 1:500 dilution of this antibody detected Nitrotyrosine on 10 μg of Nitrotyrosine Immunoblot Controls (cat. # 12-354).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
