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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Po, Rb, Sh | FC, ICC | M | AlexaFluor®488 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB5386X |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Nerve Growth Factor Receptor Antibody, p75, Alexa Fluor® 488 |
| Background Information | The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Presentation | Liquid. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Nerve Growth Factor Receptor Antibody, p75, Alexa Fluor 488 is validated for use in FC, IC for the detection of Nerve Growth Factor Receptor. |
| Key Applications |
|
| Application Notes | Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Human melanoma cell line. |
| Clone | ME20.4 |
| Host | Mouse |
| Specificity | Reacts with the low affinity nerve growth factor receptor (NGFR p75). The epitope recognized by the antibody maps within the third or fourth cysteine-rich repeats of the NGFR molecule. Reactivity has not been observed with rat, chicken, or mouse NGFR. |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. |
| Gene Symbol |
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| Non-Reactive Species |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P08138 # Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SIZE: 427 amino acids; 45183 Da SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DOMAIN: SwissProt: P08138 Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. & O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. & Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. & Contains 4 TNFR-Cys repeats. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Aliquot and store at -20 ºC, or glycerol (ASC grade or better) can be added (1:1) and the antibody stored at -20ºC for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |