Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | FC, IHC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4072 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-BrdU Antibody, clone 131-14871 |
| Alternate Names |
|
| Background Information | The thymidine analog 5-bromo-2′-deoxyuridine (BrdU) is a common reagent used for both cell proliferation assays and for the detection of apoptotic cells. BrdU is readily incorporated into newly synthesized DNA, labeling cells that have progressed through the S-phase of the cell cycle and thereby serving as a marker for proliferating cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Liquid in 10 mM Phosphate buffer, pH 7.4 containing 150 mM NaCl and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-BrdU Antibody, clone 131-14871 is a Mouse Monoclonal Antibody for detection of Bromodeoxyuridine also known as BrdU & has been validated in FC, IHC, IHC(P). This bromodeoxyuridine antibody is expect to react in all species. |
| Key Applications |
|
| Application Notes | Immunohistochemistry on deparaffinized sections of kidney, duodenum, and liver tissue: 1:1000-1:1250. Flow cytometry on 100μL of 106 cells: 1:5000. Optimal dilutions must be determined by the end user. IMMUNOHISTOCHEMICAL PROCEDURE FOR PARAFFIN EMBEDDED TISSUE SECTIONS STAINING PROCEDURE: 1. Deparaffinize sections in xylene 3 x 5 minutes. Resin embedded or GMA (Glycol methacrylate, 2-hydroxyethyl methacrylate) treated sections it is unnecessary to deparaffinize as there is no paraffin. 2. Place slides in graded alcohols (100, 95, 90%) to water at 2 minute intervals, and air dry. (Omit for GMA sections) 3. Circle sections with PAP pen or diamond pen to identify and dry thoroughly. 4. For GMA or resin sections, place slides in 0.5% Tween/PBS (pH 7.6) for 29 minutes. 5. Incubate in prewarmed (40°C) 1N HCl for 1 hour. 6. Wash 3 x 3 minutes with PBS (pH 7.6). Slides may be held at this point and the procedure continued the following day. 7. Incubate in 1X Trypsin solution (0.02-0.05% w/v, prewarmed to 40°C) for 20 minutes at room temperature for NBF (normal buffered formalin) fixed tissue or 10 minutes for Carnoys fixed tissue. 8. Wash 3 x 5 minutes with PBS (pH 7.6). 9. Incubate sections in 1% H2O2 (10 mL 30% H2O2 in 290 mL methanol) for 20 minutes, or GMA sections in 3% H2O2 (10 mL 30% H2O2 in 90 mL water) for 3 minutes. 10. Wash 2 x 2 minutes with PBS (pH 7.6). 11. Using the Vector ABC kit, add 3 drops of normal horse serum to 10 mL of PBS. Use this solution to block the slides for 20 minutes at room temperature. 12. Blot the slides of excess normal horse serum and incubate with MAB4072 diluted in PBS/BSA/Tween 20 for 1 hour at room temperature. 13. Wash 3 x 3 minutes with PBS (pH 7.6). 14. Add 3 drops of normal horse serum to 10 mL of PBS and then add 1 drop of biotinylated antibody stock. Incubate the slides with this diluted second antibody for 30 minutes at room temperature. 15. Prepare the Vectastain ABC Reagent by adding 2 drops of reagent A to 5 mL of PBS. Then add 2 drops of Reagent B to the same mixing bottle. Allow to sit for about 30 minutes prior to use. 16. Wash 3 x 3 minutes with PBS (pH 7.6). 17. Incubate the slides with ABC Reagent for 30 minutes at room temperature. 18. Wash 3 x 3 minutes with PBS (pH 7.6). 19. Prepare the substrate by mixing an equal volume of 0.02% H2O2 (made in distilled water from a 30% stock solution) and 0.1% DAB made in 0.1 M Tris buffer, pH 7.2. The H2O2 should be freshly prepared from concentrated stock. Because many peroxide substrates are unstable in the presence of H2O2 or when exposed to light, the substrate should be prepared just prior to use. Since DAB is a suspected carcinogen, care should be taken in handling and disposing of all peroxidase substrates. 20. Stain the slides with DAB substrate for 2-5 minutes. 21. Wash 2 x 2 minutes with distilled water. 22. Counterstain with hemotoxylin, dehydrate through xylene, and mount the coverslip. |
| Biological Information | |
|---|---|
| Clone | 131-14871 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes BrdU (Bromodeoxyuridine). |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein A Purfied |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |