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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, H, M, R | ICC, IHC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3878 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-MyoD1 Antibody |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MyoD1 Antibody is a high quality Mouse Monoclonal Antibody for the detection of MyoD1 & has been validated in ICC, IHC, IP & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1 μg/mL. Reacts with the ~45 kD protein. Immunohistochemistry on frozen and formalin-fixed, paraffin embedded tissue: 2-4 μg/mL. Immunocytochemistry Immunoprecipitation: 2 μg/mg of protein lysate Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein from mouse MyoD1. |
| Clone | 5.2F |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes MyoD1. The epitope is localized to amino acids 3-56 in the N-terminus of mouse MyoD1 protein. |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P15172 # Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity). SIZE: 319 amino acids; 34490 Da SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1. SUBCELLULAR LOCATION: Nucleus. PTM: Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity). & Ubiquitinated on the N-terminus; which is required for proteasomal degradation. SIMILARITY: SwissProt: P15172 ## Contains 1 basic helix-loop-helix (bHLH) domain. |
| Molecular Weight | ~ 45 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |