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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ELISA, WB, ICC | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3662 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-TIF1 beta Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Ascites |
| Presentation | Ascites. Liquid. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-TIF1 beta Antibody is a Mouse Monoclonal Antibody for detection of Transcriptional Intermediary Factor 1 also known as TIF1 beta & has been validated in ELISA, WB, ICC. |
| Key Applications |
|
| Application Notes | Western blot: 1:500-1:5,000 Immunocytochemistry: 1:500-1:5,000 ELISA: 1:500-1:5,000 Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein from mouse TIF1b. |
| Host | Mouse |
| Specificity | Recognizes transcriptional intermediary factor 1beta (TIF1beta). The epitope is localized between amino acids 123-834 of mouse TIF1beta. No cross reactivity with TIF1alpha. |
| Isotype | IgG2b |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q13263 # Forms a complex with a KRAB-domain transcription factor and increases the efficiency of KRAB-mediated repression. Silences transcription through an interaction with HP1 proteins. Acts as a corepressor of transcription for the KRAB zinc finger proteins and as a moderator of the repression activity. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of the Alpha-1-acid glycoprotein gene (By similarity). SIZE: 835 amino acids; 88550 Da SUBUNIT: Associated with HP1 alpha (CBX5), beta (CBX1) and gamma (CBX3) in interphase nuclei (By similarity). Interacts with NCOR1 and CHD3. Interacts with SETDB1, ZFP53 and ZFP68. Interacts with CEBPB and NR3C1 (By similarity). Interacts directly with CBX5 via the PxVxL motif. SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Associated with centromeric heterochromatin during cell differentiation through CBX1 (By similarity). DOMAIN: SwissProt: Q13263 The HP1 box is both necessary and sufficient for HP1 binding. The RING finger domain and the B-box domains mediate interaction with CEBPB. The PHD domain enhances the CEBPB transcriptional activity (By similarity). & Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: Belongs to the TRIM/RBCC family. & Contains 2 B box-type zinc fingers. & Contains 1 bromo domain. & Contains 1 PHD-type zinc finger. & Contains 1 RING-type zinc finger. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |