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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, Ht, M, R, Fe | WB, IHC | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB366 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-MAP1B Antibody, clone AA6 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Ascites |
| Presentation | Ascites. Liquid containing 15mM sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MAP1B Antibody, clone AA6 detects level of MAP1B & has been published & validated for use in WB, IH. |
| Key Applications |
|
| Application Notes | Immunoblotting at 1:300-1:500. Immunohistochemistry Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Rat brain microtubule-associated protein. |
| Clone | AA6 |
| Host | Mouse |
| Specificity | Reacts specifically with MAP1B (MAP5) by immunoblot. Shows selective staining of dendritic trees throughout the brain. No cross-reactivity observed to other MAPs or tubulin. Monoclonal Anti-MAP1b is immunospecific for MAP1b (also known as MAP5, MAPIX or MAP1.2; Riederer et al., 1986; Tucker et al., 1988) as determined by an immunoblot assay. Addition of antibody to microtubule proteins before polymerization results in abnormally short (but otherwise morphologically normal) microtubules. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P46821 # The function of brain MAPS is essentially unknown. Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B Binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. SIZE: 2468 amino acids; 270620 Da SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. LC1 interacts with the amino- terminal region of MAP1B. Interacts with ANP32A (By similarity). DOMAIN: SwissProt: P46821 Has a highly basic region with many copies of the sequence KKEE and KKEI/V, repeated but not at fixed intervals, which is responsible for the binding of MAP1B to microtubules. PTM: LC1 is generated from MAP1B by proteolytic processing. & Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: Belongs to the MAP1 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |