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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Ma, Ech, Gr, Su | WB, ICC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3582 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Fascin Antibody, clone 55K2 |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Fascin Antibody, clone 55K2 is validated for use in WB, IC, IH(P) for the detection of Fascin. |
| Key Applications |
|
| Applications Not Recommended |
|
| Application Notes | Western blot. Recognizes a protein of 55 kDa. Suggested working dilution is 1:100-1:1,000. Suggested blocking buffer is 3% BSA in PBS or 3% non fat milk in PBS. Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000. Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Human fascin |
| Clone | 55K2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Fascin. The antibody is strongly positive when antigen-presenting dendritic cells are matured. MAB3582 is a sensitive marker for Reed-Sternberg Cells of Hodgkins Disease. The protein is an actin bundling protein specifically localized in filopodia and growth cones. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q16658 # Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers. SIZE: 493 amino acids; 54530 Da SUBUNIT: Associates with beta-catenin. TISSUE SPECIFICITY: Ubiquitous. PTM: Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin. DISEASE: SwissProt: Q16658 # Marks and mediates breast cancer metastasis to the lungs. FSCN1 is not functionally validated but achieves the highest statistical significance (P less than 0.000001). Those subjects expressing the lung metastasis signature have a significantly poorer lung metastasis-free survival, but not bone metastasis-free survival, compared to subjects without the signature. SIMILARITY: SwissProt: Q16658 ## Belongs to the fascin family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |