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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, H, M, R | IHC | M | AlexaFluor®488 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3418X |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-MAP2 Antibody, Alexa Fluor® 488 conjugated |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Control |
|
| Presentation | Purified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-MAP2 Antibody, Alexa Fluor 488 conjugated is validated for use in IH for the detection of MAP2. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Optimal working dilutions must be determined by end user. APPLICATION NOTES: Immunohistochemistry: Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde. |
| Biological Information | |
|---|---|
| Immunogen | Bovine brain microtubule protein. |
| Clone | AP20 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes MAP-2 (microtubule associated protein-2). MAP-2 is one of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (1). MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (2,3). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (4,5). SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. SIZE: 1827 amino acids; 199539 Da PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |