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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3321 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-MMP-13 Antibody, clone 181-15A12 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin to recombinant human matrix metalloproteinase 13 (human MMP-13, procollagenase-3). Liquid in 0.1 M PBS, pH 7.0 containing 4% protease free bovine serum albumin. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MMP-13 Antibody, clone 181-15A12 detects level of MMP-13 & has been published & validated for use in ELISA, WB, IH(P). |
| Key Applications |
|
| Application Notes | Immunoblotting Immunohistochemistry on paraffin-embedded tissue sections. |
| Biological Information | |
|---|---|
| Clone | 181-15A12 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with precursor and active forms of human MMP-13 and does not cross-react with human MMP-1, 2, 3, 7, 8, 9. This is a purified mouse moncolonal antibody to recombinant human matrix metalloproteinase 13 (human MMP-13, procollagenase-3). |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P45452 # Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit. SIZE: 471 amino acids; 53820 Da TISSUE SPECIFICITY: Seems to be specific to breast carcinomas. DOMAIN: SwissProt: P45452 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. DISEASE: SwissProt: P45452 # Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia type 2 (SEMD2) [MIM:602111]; also known as spondyloepimetaphyseal dysplasia type Missouri. SEMDs are a heterogeneous group of skeletal disorders characterized by defective growth and modeling of the spine and long bones. The SEMDs are distinguished from the spondylometaphyseal dysplasias and the spondyloepiphyseal dysplasias by the combined involvement of the epiphyses and metaphyses. The 3 disorders have malformations of the vertebrae in common. SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains. |
| Product Usage Statements | |
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| Availability by Geography |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |