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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, B, Gp, Mk | WB, IH(P), EM, ACT | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB331-C |
| Replaces | MAB331 |
| Description | Anti-SNAP-25 Antibody, clone SP14 (Ascites Free) |
| Alternate Names |
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| Background Information | Synaptosomal-associated protein 25 (UniProt P60880; also known as SNAP-25, SUP, Super protein, Synaptosomal-associated 25 kDa protein) is encoded by the SNAP25 (also known as SNAP) gene (Gene ID 6616) in human. SNAP-25 belongs to the superfamily of soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor (SNARE) proteins involved in mediating vesicle fusion events. Target or t-SNAREs located in the membranes of target compartments form stable subcomplexes and engage vesicle or v-SNAREs in the membranes of transport vesicles to facilitate the fusion. SNAP-25 is a t-SNARE protein of the trans-SNARE complex that executes fusion between the synaptic vesicle and plasma membrane. SANP-25 does not have a transmembrane domain and is anchored to the cytosolic face of membranes via palmitoyl side chains covalently linked to cysteine residues. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SNAP-25 Antibody, clone SP14 (Ascites Free) is an antibody against SNAP-25 for use in Western Blotting, Immunohistochemistry (Paraffin), Electron Microscopy and Activity Assay. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1.0 µg/mL from a representative lot detected SNAP-25 in 10 µg of SHSY-5Y cell lysate. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SNAP-25 in human cerebellum, mouse cerebellum, and mouse cerebral cortex tissue. Immunohistochemistry Analysis: A representative lot localized SNAP-25 immunoreactivity in human and rat brain vibratome sections by fluorescent immunohistochemistry (Garbelli, R., et al. (2008). J. Comp. Neurol. 506(3):373-386). Immunohistochemistry Analysis: A representative lot immunostained axons in the white matter at the thalamus border using paraffin-embedded tissue sections from a diseased patient with fatal familial insomnia (FFI) due to a prion protein gene mutation (Sasaki, K., et al. (2005). Neuropathol. Appl. Neurobiol. 31(1):80-87). Immunohistochemistry Analysis: A representative lot detected SNAP-25 immunoreactivity associated with varicosities, but not axon terminals in the adjacent superficial layers of the spinal cord dorsal horn using tissue samples from guinea pigs, mice, and toads (Morris, J.L., et al. (2005). J. Comp. Neurol. 483(1):1-16). Immunohistochemistry Analysis: A representative lot detected SNAP-25 immunoreactivity in intragemmal nerve processes and among a small subset of taste cells in rat circumvallate papillae tissue sections (Yang, R., et al. (2000). J. Comp. Neurol. 424(2):205-215). Western Blotting Analysis: A representative lot detected downregulated frontal cortex SNAP-25 among rats subjected to stress by a 3-hr decapicone restraint, and upregulated frontal cortex SNAP-25 among rats subjected to daily basolateral amygdala (BLA) Urocortin 1 (Ucn1) via bilateral i.c. injection (Ray, B., et al. (2011). Neuroscience. 184(16):139-150). Western Blotting Analysis: A representative lot detected greatly reduced brain SNAP-25 level among Tg2576 transgenic mice harboring human APP695 double Swedish mutation (App Tg mice). The brain SNAP-25 levels were greatly recovered among App Tg mice received aged garlic extract (AGE) or S-allyl-L-cysteine (SAC) treatment (Ray, B., et al. (2011). J. Neurochem. 117(3):388-402). Western Blotting Analysis: A representative lot detected upregulated SNAP-25 level in cerebral cortical samples from diazoxide-treated 3xTgAD mice than from untreated mice (Liu, D., et al. (2010). J. Alzheimers Dis. 22(2):443-573). Western Blotting Analysis: A representative lot detected Rivastigmine treatment-induced SNAP-25 upregulation in a dose-dependent manner using primary embryonic rat cortical cultures (Bailey, J.A., and Lahiri, D.K. (2010). J. Neurochem. 112(4):843-853). Western Blotting Analysis: A representative lot detected calpain-mediated SNAP-25 cleavage in murine cerebral cortex synaptosome preparations (Grumelli, C., et al. (2008). Mol. Cell. Neurosci. 39(3):314-323). Western Blotting Analysis: A representative lot detected similar prefrontal cortex SNAP-25 levels among samples from chronic alcoholics and non-alcoholic individuals (Henriksson, R., et al. (2008). Synapse. 62(11):829-833). Western Blotting Analysis: A representative lot detected SNAP-25 in human and rat cerebral cortex homogenates (Garbelli, R., et al. (2008). J. Comp. Neurol. 506(3):373-386). Western Blotting Analysis: A representative lot detected upregulated SNAP-25 in the Brodmanns area 9 (BA9) region of frontal cortex from patients with bipolar I disorder (BPDI), but not those with schizophrenia (Scarr, E., et al. (2006). Bipolar Disord. 8(2):133-143). Western Blotting Analysis: Representative lots detected multiples immunoreactive bands corresponding to free SNAP-25 (~25 kDa), and SNAP-25-containing SNARE complexes (~100 and ~230 kDa) in unboiled rat phaeochromocytoma PC12 whole cell or membrane SDS extracts, while only uncomplexed SNAP-25, but not SDS-resistant SNARE complex, was detected in boiled extracts (Kubista, H., et al. (2003). J. Cell Sci. 117(Pt 6):955-966; Hussl, S., et al. (2007). Purinergic Signal.3(4):367-375). Western Blotting Analysis: A representative lot detected SNAP-25 in human cerebral spinal fluid (CSF) samples, as well as in rat and human brain homogenates (Thompson, P.M., et al. (1998). J. Psychiatr. Res. 32(5):297-300). Electron Microscopy Analysis: A representative lot localized SNAP-25 immunoreactivity in rat cerebellum sections, as well as in human and rat cerebral cortex sections (Garbelli, R., et al. (2008). J. Comp. Neurol. 506(3):373-386). Activity Assay: A representative lot reduced K1 depolarization-induced GABA and glutamate release from rat brain cortex synaptosomes (Raiteri, M., et al. (2000). J. Neurochem. 74(1):423-431). ELISA Analysis: A representative lot detected SNAP-25 immunoreactivity by ELISA (Honer, W.G., et al. (1993). Brain Res. 609(1-2):9-20). |
| Biological Information | |
|---|---|
| Immunogen | Human brain proteins immunoprecipitated with EP10 (Cat. No. MAB332). |
| Epitope | N-terminal half |
| Clone | SP14 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone SP41 recognizes both SNAP-25 isoforms A & B. Clone SP41 is reactive toward endogenous or exogenously expressed SNAP-25 in COS cells, but not SNAP-25 expressed in E. coli, indicative of a posttranslational modification-dependent antigenicity. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~25 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human brain tissue lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected SNAP-25 in 10 µg of human brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |