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Millipore Detect The Glutathione: N-Ethylmaleimide Adduct Protein Using This Anti-Glutathione: N-Ethylmaleimide Adduct, Clone 8.1gsh Validated For Use In Elisa, Flow, Icc, Ihc & Wb. - Mill (Additional S&H or Hazmat Fees May Apply)
Detect the Glutathione: N-ethylmaleimide adduct protein using this Anti-Glutathione: N-ethylmaleimide adduct, clone 8.1GSH validated for use in ELISA, Flow, ICC, IHC & WB.
Immunocytochemistry: 1:100 - 1:500 for assessment of intracellular GSH distribution.
ELISA: 1:100,000 - 1:200,000 to quantify GSH at >2 mM in biological specimens.
Flow cytometry: 1:100 - 1:1,000
Cells were fixed with cold 1% PFA for 5 min and vortexed into methanol (-20°C) containing 5mM NEM (10 min on dry ice) or 10 μM NEM (N-ethyl maleimide) for 30 min at RT.
Optimal working dilutions must be determined by end user.
Positive Control: Peripheral blood mononuclear cells (PBMC) treated with NEM
Biological Information
Immunogen
GS-NEM conjugated to KLH
Clone
8.1GSH
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Reactive with thiol-modified glutatione. Does not react with oxidized glutathione (GSSG) or glutathione (GSH) in mixed disulfide linkage with proteins. MAB3194 is specific to the glutathione:N-ethylmaleimide adduct; thus it is useful for quantification of reduced glutathione (GSH) after reaction with N-ethylmaleimide. For use in ELISA or flow cytometry, sample must first be reacted with NEM.
Isotype
IgG1
Species Reactivity
Mammals
Antibody Type
Monoclonal Antibody
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
