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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ca, Gt, H, Po, Rb, Fe | ELISA, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3152 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cardiac Troponin I Antibody, a.a. 87-91, clone 8E10 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid in PBS (pH 7.4) with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Cardiac Troponin I using this Anti-Cardiac Troponin I Antibody, a.a. 87-91, clone 8E10 validated for use in ELISA & WB. |
| Key Applications |
|
| Application Notes | Western blotting: identifies two bands 14kDa and 18kDa which appear to be breakdown products of full length cTn1 (Morjana N. 1998, Degradation of human cardiac troponin I after myocardial infarction. Biotechnol Appl. Biochem. 28:105-11). EIA: MAB3152 has been used successfully in ELISA and immunoaffinity purification. Its affinity constant is 8x10(10). It can be used with MAB3150 in sandwhich ELISA as the coating antibody. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Free human cardiac troponin 1. |
| Epitope | a.a. 87-91 |
| Clone | 8E10 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | MAB3152 reacts equally with free cardiac troponin 1 (cTn1) and cTn1 forming complexes with other troponin components. It recognizes epitopes that are not perturbed or sterically shielded by other troponin components. Not affected by heparin, phosphorylation, oxidation and complex formation. Recognizes an epitope located between amino acid residues 87 and 91 of cTnI. There is no cross-reaction with skeletal muscle troponin 1. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Troponin I (TnI) is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle; see also TNNI1 (MIM 191042). The others are troponin T (TnT; see MIM 191041) and troponin C (TnC; see MIM 191040).[supplied by OMIM] |
| Gene Symbol |
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| Non-Reactive Species |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P19429 # Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. SIZE: 210 amino acids; 24008 Da SUBUNIT: Binds to actin and tropomyosin. Interacts with TRIM63. DISEASE: SwissProt: P19429 # Defects in TNNI3 are the cause of familial hypertrophic cardiomyopathy type 7 (CMH7) [MIM:191044]; also designated FHC or HCM. Familial hypertrophic cardiomyopathy [MIM:192600] is a heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The prevalence of the disease in the general population is 0.2%. FHC is clinically heterogeneous, with inter- and intrafamilial variations ranging from benign to malignant forms with a high risk of cardiac failure and sudden cardiac death. & Defects in TNNI3 are the cause of familial restrictive cardiomyopathy (RCM) [MIM:115210]. RCM is an heart muscle disorder characterized by impaired filling of the ventricles with reduced volume in the presence of normal or near normal wall thickness and systolic function. The disease may be associated with systemic disease but is most often idiopathic. SIMILARITY: SwissProt: P19429 ## Belongs to the troponin I family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |