Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ma | FC, ICC, IF, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB312R |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-A2B5 Antibody, clone A2B5-105 |
| Alternate Names |
|
| Background Information | A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors and neuroendocrine cells. The A2B5 antigen is present in type 2 astrocytes but absent in type 1 astrocytes, allowing for the differentiation of these different types of cells. A2B5 antibody has been used in flow cytometry and immunohistochemistry experiments to stain type II astrocytes, cells involved in gliogenesis and cells committed to the oligodendrocyte lineage. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Liquid. Buffer = PBS, pH 7.1 with 2 mg/mL BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-A2B5 Antibody, clone A2B5-105 detects level of A2B5 & has been published & validated for use in FC, IC, IF, IH. |
| Key Applications |
|
| Application Notes | Immunocytochemistry: 5-10 μg/mL Immunohistochemistry: 5-10 μg/mL (Levison & McCarthy, 1989) Complement-mediated cytotoxicity (Eisenbarth et al., 1979) Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Embryonic chicken retinal cells |
| Clone | A2B5-105 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes an epitope common to silaogangliosides and sulfatides from the plasma membranes of neurons, and neuroendocrine and glial cells. Anti-A2B5 has been used to specifically stain: Neurofibrillary tangles in the post-mortem brain tissue of patients with a confirmed diagnosis of Alzheimers disease (Majocha et al., 1989) Neurons of chicken retina, brain, spinal cord, and dorsal root ganglia (Schnitzer & Schachner, 1982; Eisenbarth et al., 1979). Rat striatal nerve terminals [synaptosomes (Wolf & Kapatos, 1989)]. Neuroblastoma cells in man (Oppedal et al., 1989; Coakham et al., 1985). Rat insulinoma cells and pancreatic islets cells (Shimizu et al., 1983; Bartholomeusz et al., 1989). Human and rat thymic epithelial cells (Haynes et al., 1988). Cells involved in gliogenesis (Bottenstein et al., 1988; Suzumura & Slerberg, 1989). Oligodendrocytes and astrocytes, type II (Dubois-Dalcq et al., 1990). |
| Isotype | IgM |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
|
| Purification Method | Ammonium Sulfate Precipitation |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain the lyophilizate frozen at -20°C. Store the reconstituted antibody solution at -20°C for 12 months in undiluted aliquots. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |