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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3034 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-DNA Antibody, single stranded, clone 16-19 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | 0.1 M Tris-Glycine (pH 7.4), 150 nM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-DNA Antibody, single stranded, clone 16-19 is an antibody against DNA for use in ELISA. |
| Key Applications |
|
| Application Notes | ELISA This antibody is evaluated using ELISA on single stranded DNA from Calf Thymus. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Epitope | single stranded DNA |
| Clone | 16-19 |
| Host | Mouse |
| Specificity | Single stranded DNA (ssDNA) (poly-deoxythymidine). Shows 90% reactivity to ssDNA and 12% reactivity to dsDNA. ANTIGEN BINDING PROFILE: Relative Binding To: Natural DNA Synthetic homopolymers dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU) 0.03 0.84 0.02 0.11 0.03 1.00 0.03 0.53 a) Native calf thymus DNA b) Heat-denatured calf thymus DNA Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 μg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0). BINDING ASSAY Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) for one hour at room temperature, followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation. ISOELECTRIC POINT = 8.3 |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |