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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IHC | AHm | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2637 |
| Description | Anti-Integrin beta-3 (CD61) Antibody, clone HMb3-1 |
| Alternate Names |
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| Background Information | The integrin family of cell adhesion receptors consists of several membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Platelet membrane glycoprotein Integrin beta3 or CD61 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified armenian hamster monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Integrin beta-3 (CD61) using this Anti-Integrin beta-3 (CD61) Antibody, clone HMb3-1 validated for use in FC, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Piali, 1995). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to mouse Integrin beta-3. |
| Epitope | Unknown |
| Clone | HMb3-1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Armenian Hamster |
| Specificity | The antibody recognizes Integrin beta-3. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. SUBUNIT STRUCTURE: Heterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIB or alpha-V. Interacts with FLNB and COMP (By similarity). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. PTM: Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling (By similarity). SEQUENCE SIMILARITIES: Belongs to the integrin beta chain family. Contains 1 VWFA domain. |
| Molecular Weight | ~ 86-87 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry on mouse bone marrow-derived cells. Flow Cytometry Analysis: 0.1-1 µg/mL of the antibody detected Integrin beta-3 on mouse bone marrow-derived cells |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |