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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IP, ICC, IHC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2627 |
| Description | Anti-VCAM-1 (CD106) Antibody, clone 6C7.1 |
| Alternate Names |
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| Background Information | Vascular cell adhesion protein 1 (VCAM-1) is a cell surface adhesion molecule belonging to the immunoglobulin cell-cell adhesion receptor family and is comprised of seven Ig-like C2-type domains. VCAM-1 is a crucial component in cell-cell recognition and seems to be involved in cellular adhesion between endothelial cells and leukocytes. It has a regulatory role for both signal transduction and adhesion through its interaction with leukocyte beta-1 integrin VLA4, an interaction that is potentially involved in immune and inflammatory emigration responses of leukocytes. Studies have shown upregulation of VCAM-1 to be critical in responding to inflammatory diseases such as atherosclerosis. VCAM-1 is primarily expressed in endothelial cells and is expressed as two known isoforms found within the cell membrane. Isoform 1 functions as a single-pass type 1 membrane protein, while Isoform 2 acts as a GPI-anchor. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rat monoclonal IgG1κ in buffer containing PBS without preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-VCAM-1 (CD106) Antibody, clone 6C7.1 is an antibody against VCAM-1 (CD106) for use in FC, IP, IC, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A previous lot was used by independent laboratory in IH. (Norman, U., et al. (2008). Journal of Leukocyte Biology. 84:68-76.) Immunocytochemistry Analysis: A previous lot was used by independent laboratory in IC. (Norman, U., et al. (2008). Journal of Leukocyte Biology. 84:68-76.) |
| Biological Information | |
|---|---|
| Immunogen | Mouse endothelial cells |
| Clone | 6C7.1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G |
| UniProt Number |
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| UniProt Summary | FUNCTION: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. SUBUNIT STRUCTURE: Binds to ECMV-D capsid proteins and acts as a receptor for this virus. SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Lipid-anchor › GPI-anchor. TISSUE SPECIFICTY: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow. PTM: The GPI-anchor is located on position 319 of isoform 2. SEQUENCE SIMILARITIES: Contains 7 Ig-like C2-type (immunoglobulin-like) domains. |
| Molecular Weight | 81 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in mouse bone marrow cells. Flow Cytometry Analysis: 10 µg of this antibody detected VCAM-1 on mouse bone marrow cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |