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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, IP, IHC, FC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2624 |
| Description | Anti-Endomucin Antibody, clone V.5C7 |
| Alternate Names |
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| Background Information | Endomucin is an endothelial sialomucin belonging to a class of glycoproteins characterized by their high levels of O-glycosolation and rich quantities of sialic acid. It inhibits focal adhesion assembly and hinders extracellular matrix and cell interaction. Observed expression is highest in kidney heart, brain, liver, lung, spleen, and thymus. Endomucin is only found in endothelial cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Endomucin Antibody, clone V.5C7 detects level of Endomucin & has been published & validated for use in WB, IP, IH, FC. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP of bEND cell lysate (Morgan, 1999). Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in immunhistochemical staining of frozen and paraffin-embedded tissue sections of mouse tongue, heart, uterus, brain, and small intestine (Morgan, 1999). Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC of transfected COS-7 cells (Morgan, 1999). |
| Biological Information | |
|---|---|
| Immunogen | Mouse endothelial cells |
| Clone | V.5C7 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G |
| UniProt Number |
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| UniProt Summary | FUNCTION: Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (by similarity). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein Potential. TISSUE SPECIFICTY: Highly expressed in heart and kidney, followed by brain, spleen, thymus, liver and lung. Exclusively expressed in endothelial cells. PTM: Highly O-glycosylated. Sialic acid-rich glycoprotein. |
| Molecular Weight | ~ 75kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on mouse endothelioma cell line, bEND3, cell lysate. Western Blot Analysis: 0.0025 µg/mL of this antibody detected Endomucin in 10 µg of bEND3 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |