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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2616 |
| Description | Anti-Kindlin-1 Antibody, clone 4A5.14 |
| Alternate Names |
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| Background Information | The Kindlin family is a group of focal adhesion proteins that have recently become a point of interest because of their ability to bind and activate integrins. Abnormalities in this ability have linked Kindlin to several diseases, both inherited and acquired, such as Kindler syndrome, cancer, and leukocyte adhesion deficiency. Specifically associated with Kindler syndrome, is the isoform Kindlin-1 which plays a key role in adhering the actin cytoskeleton to the extracellular matrix of basal keratinocytes. Kindlin-1 may also be involved in tumor growth by modulating TGF-beta 1 signaling (Shi, 2007). It is most widely expressed in the brain, kidney, colon, placenta, skeletal muscle, adrenal gland, and prostate. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Kindlin-1 Antibody, clone 4A5.14 is an antibody against Kindlin-1 for use in WB, IC, IH. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Papachristou, D.J., et al. (2007). Histopathology. 51(4):499–508.) Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH. (Papachristou, D.J., et al. (2007). Histopathology. 51(4):499–508.) |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human Kindlin-1. |
| Epitope | Unknown |
| Clone | 4A5.14 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | The antibody recognizes Kindlin-1. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the fermitin family, and contains a FERM domain and a pleckstrin homology domain. The encoded protein is involved in integrin signaling and linkage of the actin cytoskeleton to the extracellular matrix. Mutations in this gene have been linked to Kindler syndrome. [provided by RefSeq]. |
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Involved in cell adhesion, possibly via its interaction with integrins. May mediate TGF-beta 1 signaling in tumor progression. SUBUNIT STRUCTURE: Interacts with the cytoplasmic domain of integrins ITGB1 and ITGB3. SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Cell junction › focal adhesion. Cell projection › ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Constituent of focal adhesions. Colocalizes with filamentous actin. Upon TGFB1 treatment, it localizes to membrane ruffles. TISSUE SPECIFICITY: Expressed in brain, skeletal muscle, kidney, colon, adrenal gland, prostate, and placenta. Weakly or not expressed in heart, thymus, spleen, liver, small intestine, bone marrow, lung and peripheral blood leukocytes. Overexpressed in some colon and lung tumors. In skin, it is localized within the epidermis and particularly in basal keratocytes. INDUCTION: By TGF-beta 1. DOMAIN: The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain. INVOLVEMENT IN DISEASE: Defects in FERMT1 are the cause of Kindler syndrome [MIM:173650]. Kindler syndrome is an autosomal recessive disorder characterized by neonatal blistering, sun sensitivity, atrophy, abnormal pigmentation and fragility of the skin. SEQUENCE SIMILARITIES: Belongs to the kindlin family. Contains 1 FERM domain. Contains 1 PH domain. |
| Molecular Weight | ~ 70 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/ml of this antibody detected Kindlin-1 in 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |