Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2613 |
| Description | Anti-UPAR-Associated Protein Antibody, clone 2.h.9:F12 |
| Alternate Names |
|
| Background Information | Urokinase plasminogen activator receptor-associated protein (uPARAP/Endo180/MRC2) is a member of the macrophage mannose receptor family of endocytic transmembrane glycoproteins. UPAR-Associated Protein is a membrane protein involved in matrix turnover during tissue remodeling, particularly the uptake and intracellular degradation of collagen, (Sulek, 2007). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-UPAR-Associated Protein Antibody, clone 2.h.9:F12 is an antibody against UPAR-Associated Protein for use in WB, IH. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | uPAR D3-tagged recombinant protein corresponding to human UPAR-Associated Protein. |
| Epitope | Unknown |
| Clone | 2.h.9:F12 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | The antibody recognizes UPAR-Associated Protein. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | MRC2 is a recycling endocytic receptor that functions in cell motility and remodeling of the extracellular matrix by promoting cell migration and uptake of collagens for intracellular degradation (Wienke et al., 2007 [PubMed 17974964]).[supplied by OMIM]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: May play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix co-operating with the matrix metalloproteinases (MMPs). SUBUNIT STRUCTURE: Interacts with C-terminal region of type I collagen/COL1A1 By similarity. Interacts directly with PLAUR/UPAR and PLAU/pro-UPA to form a tri-molecular complex. Interacts with collagen V. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Ubiquitous with low expression in brain, placenta, lung, kidney, pancreas, spleen, thymus and colon. Expressed in endothelial cells, fibroblasts and macrophages. Highly expressed in fetal lung and kidney. DOMAIN: C-type lectin domains 3 to 8 are not required for calcium-dependent binding of mannose, fucose and N-acetylglucosamine. C-type lectin domain 2 is responsible for sugar-binding in a calcium-dependent manner. Fibronectin type-II domain mediates collagen-binding. Ricin B-type lectin domain contacts with the second C-type lectin domain By similarity. PTM: Phosphorylated. N-glycosylated. SEQUENCE SIMILARITIES: Contains 8 C-type lectin domains. Contains 1 fibronectin type-II domain. Contains 1 ricin B-type lectin domain. |
| Molecular Weight | ~ 180 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in U937 cell lysate. Western Blot Analysis: 0.2 µg/ml of this antibody detected UPAR-Associated Protein on 10 µg of U937 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |