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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2610 |
| Description | Anti-Zyxin Antibody, clone 14E11.1 |
| Alternate Names |
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| Background Information | Zyxin, is a cytosolic, focal adhesion protein belonging to the Ajuba family and containing 3 LIM zinc-binding domains. It associates with the actin cytoskeleton near the adhesion plaques and, during mitosis, with microtubules. Zyxin localizes to stress fibers, cell-cell junctions and colocalizes with vinculin, paxillin and VASP at focal adhesions but not at the tips of lamellipodia. Zyxin is involved in the regulation of actin dynamics and is a likely component of the signal transduction pathway mediating adhesion-stimulated changes in gene expression. Zyxin displays an actin nucleating activity that is dependent on its amino terminal proline-rich motifs and independent of Arp2/3 complex. Zyxin appears to function as an adaptor or docking protein to organize protein complexes important for regulated actin cytoskeleton dynamics. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Zyxin Antibody, clone 14E11.1 is validated for use in WB, IC for the detection of Zyxin. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Zyxin in NIH/3T3, A431, and HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Zyxin. |
| Clone | 14E11.1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgMκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq]. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression By similarity. SUBUNIT STRUCTURE: Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH, EVL and VASP. Interacts with the first LIM domain of TES. SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › cytoskeleton. Nucleus. Cell junction › focal adhesion. Note: Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1. SEQUENCE SIMILARITIES: Belongs to the zyxin/ajuba family. Contains 3 LIM zinc-binding domains. |
| Molecular Weight | ~84 kDa observed. A non-specific band may be observed at ~36 kDa. The calculated molecular weight is 61 kDa. Zyxin is a phosphoprotein with an apparent molecular weight of 82–84 kDa (van der Gaag, E., et al. (2002). Journal of Investigative Dermatology. 118:246–254.). As a substrate of caspases, Zyxin can be cleaved as 30, 37, 48 and 70 kDa. (C-B Chan, C-B., et al. (2007). Cell Death and Differentiation. 14:1688–1699.) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HUVEC cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Zyxin in 10 µg of HUVEC cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |