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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | ICC, IF | M | AlexaFluor®488 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2300X |
| Trade Name |
|
| Description | Milli-Mark™ FluoroPan Neuronal Marker - Alexa488 conjugated |
| Background Information | Antibodies to neuronal proteins have become critical tools for identifying neurons and discerning morphological characteristics in culture and complex tissue. While the labeling from classic histological techniques, such as Golgi staining, and modern molecular approaches, such as GFP constructs, yield excellent cytoarchitectural detail, these approaches are technically challenging and impractical for many neuroscience research needs. Neuron-specific antibodies are convenient precision tools useful in revealing cytoarchitecture, but are limited to the protein target distribution within the neuron, which may differ greatly from nucleus to soma to dendrite and axon. To achieve as complete a morphological staining as possible across all parts of neurons, Millipore previously developed a monoclonal antibody blend (Catalog No. MAB2300) that reacts against key somatic, nuclear, dendritic, spine, and axonal proteins distributed across the pan-neuronal architecture. Now Millipore has formulated MAB2300X, an Alexa 488 conjugated version of the pan-neuronal antibody blend MAB2300 to facilitate immunofluorescence studies. Each antibody within the blend is conjugated to Alexa 488 therefore enabling detection of an entire neuron with only one microscopic emission channel. MAB2300X has been validated in diverse fixations, cell culture, and immunohistochemistry protocols, giving researchers a convenient and specific qualitative and quantitative tool for studying neuronal morphology. High morphological resolution has been achieved using MAB2300x including distral dentrites and spines. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Control |
|
| Presentation | Blended monoclonal antibody cocktail in PBS with 0.05% NaN3 |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Milli-Mark FluoroPan Neuronal Marker -Alexa488 conjugated is an antibody targeting the FluoroPan Neuronal Marker protein, validated for use in ICC & IF. |
| Key Applications |
|
| Application Notes | Immunocytochemistry: 1. Culture neurons as desired. When ready, gently wash with PBS and fix cells with 4% paraformaldehyde in PBS, 30min at room temp (RT). 2. Wash with PBS, 3 x 5min each, at RT and block with blocking buffer (1% BSA, 5% Serum, 0.2% Triton X in PBS) for 1 hr at RT. 3. Incubate cells with Milli-Mark FluoroPan Neuronal Marker (Mouse IgG conjugated with Alexa 488) for 2hr at RT (dilute antibody in blocking buffer). 4. Wash with PBS, 3 x 5min each, and cover slip with LIGHT DIAGNOSTICS Mounting Fluid (Catalog No. #5013). View using fluorescent microscope with FITC filter. |
| Biological Information | |
|---|---|
| Epitope | Whole Neuron Marker |
| Host | Mouse |
| Specificity | MAB2300X is specific to axons (neurites), dendrites, spines, nucleus, and the cell body of neurons. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Rat, Mouse, and Human. Reactivity with other species has not been determined. |
| Antibody Type | Monoclonal Antibody |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely tested on cortical neurons in Immunocytochemistry 1:150 - 1:100. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |