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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | WB, IP, ICC, ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2263 |
| Description | Anti-GluR1-NT (NT) Antibody, clone RH95 |
| Alternate Names |
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| Background Information | Glutamate receptors (GluRs) are a diverse group responsible for mediating most of the excitatory synaptic transmission in the CNS of vertebrates. They can be categorized as ionotropic or metabotropic and subcategorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this position is occupied by a Gln residue. The insertion or removal of GluR1/GluR4 oligomeric channels from postsynaptic membranes appears to be LTP/LTD activity dependent while GluR2/GluR3 oligomers are continuously cycling. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GluR1-NT (N-terminus) Antibody, clone RH95 is an antibody against GluR1-NT (N-terminus) for use in WB, IP, IC, ELISA. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | Thyroglobulin-conjugated linear peptide of rat GluR1 designed around the N-terminus. |
| Epitope | N-terminus |
| Clone | RH95 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes GluR1 at the N-terminus. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Proven to react with rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Mouse homolog may play a role in regulation of neuronal synaptic plasticity and conditioned reinforcement learning. [RGD] |
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. SUBUNIT STRUCTURE: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with CACNG2, HIP1 and probably RASGRF2. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note: Interaction with CACNG2 promotes cell surface expression. TISSUE SPECIFICITY: Detected in cerebellum (at protein level). PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis By similarity. POLYMORPHISM: Both variants Ser-710 and Thr-710 are phosphorylated at this position. MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate. SEQUENCE SIMILARITIES: Belongs to the glutamate-gated ion channel (TC 1.A.10) family. [View classification] |
| Molecular Weight | 110 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in rat brain lysate. Western Blot Analysis: A 1:1000 dilutions of this antibody detected GluR1 in 10 µg of rat brain lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |