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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IHC, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2262 |
| Description | Anti-Excitatory amino acid transporter 2 Antibody, clone G6 |
| Alternate Names |
|
| Background Information | The activity of the main glutamate transporter in the CNS, Excitatory amino acid transporter 2 (also known as GLT1), can be regulated by protein kinase C (PKC). It is known that activation of PKC by phorbol esters promotes the clathrin-dependent internalization of the transporter, followed by its lysosomal degradation. However, the molecular mechanisms that link PKC activation and the internalization of the excitatory amino acid transporter 2 are not fully understood. The internalization process may play a role in the ubiquitylation of lysine residues located in the C-terminal tail of the excitatory amino acid transporter 2. Exposure to PMA increases the ubiquitylation of the excitatory amino acid transporter 2 in transfected cells and in the rat brain, and this ubiquitylated excitatory amino acid transporter 2 accumulates in the intracellular compartment. The activation of PKC induces the ubiquitylation of these C-terminal lysine residues in the Excitatory amino acid transporter 2 and this modification mediates the interaction of the transporter with the endocytic machinery. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Excitatory amino acid transporter 2 Antibody, clone G6 is validated for use in WB, IH, IC for the detection of Excitatory amino acid transporter 2. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 0.5 µg/ml from a previous lot detected Excitatory amino acid transporter 2 on 10 µg of human brain tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to the C-terminus of human Excitatory amino acid transporter 2. |
| Epitope | C-terminus |
| Clone | G6 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes the C-terminus of Excitatory amino acid transporter 2. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human and mouse. Predicted to react with rat based on 100% immunogen peptide sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. SUBUNIT STRUCTURE: Homotrimer. Interacts with JUB By similarity. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. PTM: Glycosylated. SEQUENCE SIMILARITIES: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. [View classification] |
| Molecular Weight | 62 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse brain tissue lysate. Western Blot Analysis: 0.5 µg/ml of this antibody detected Excitatory amino acid transporter 2 on 10 µg of mouse brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |