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Dinitrophenol (DNP) is a small water-soluble, hydrophobic ligand that binds to transthyretin at acidic pH and also reduces the formation of amyloid fibril. DNP levels of 100 mg/l or more is greatly toxic to acclimated bacteria. DNP has been associated with possible therapies for neurodegenerative and neurological disorders.
Product Information
Format
Purified
Control
Oxyblot protein standards
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-DNP Antibody, clone 9H8.1 is validated for use in WB for the detection of DNP.
Key Applications
Western Blotting
Biological Information
Immunogen
KLH-conjugated DNP
Clone
9H8.1
Host
Mouse
Isotype
IgG1κ
Species Reactivity
All
Species Reactivity Note
Demonstrated to react with a DNP moiety in a wider range of species. This antibodys reactivity is expected to be species independent.
Antibody Type
Monoclonal Antibody
Gene Symbol
DNP
Purification Method
Protein G
Molecular Weight
Various kDa observed. The Oxyblot protein standards (S7151) were used to test this antibody. These standards are phosphorylase B, BSA, OVA, Carbonic Anhydrase, and trypsin inhibitor which all contain 1-3 DNP residues per protein.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in Oxyblot protein standards.
Western Blot Analysis: 0.5 µg/mL of this antibody detected DNP on 10 µg of Oxyblot protein standards.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
