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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ELISA, IP, WB | M | Serum | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1810 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-ADAMTS-1 Antibody, clone 5D4E11B6 |
| Alternate Names |
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| Background Information | ADAMTS-1 is a member of a family of extracellular proteases known as ADAMTS (a disintegrin and metalloprotease with thrombospondin motifs). Members of the ADAMTS family share distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TSP) motif. Individual members of this family differ in the number of C-terminal TSP motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TSP motifs. ADAMTS-1 cleaves aggrecan, a cartilage proteoglycan and appears to demonstrate angiogenesis inhibitory activity. The maturation of ADAMTS-1 requires two independent and sequential processing events that may release two forms of the protein. With the cleavage of the prodomain from the 110 kDa zymogen, an 87 kDa active form remains which can be further processed by removal of the catalytic subunit from the TSP repeats, leaving the C-termianl 65 kDa soluble form. The proteolytic deletion of the last two TSP repeats, is probably significant for the in vivo function of this protein. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Ascites. Liquid containing 0.05% NaN3. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect ADAMTS-1 using this Anti-ADAMTS-1 Antibody, clone 5D4E11B6 validated for use in ELISA, IP & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1:2000 - 1:4000 dilution on A431 cell lysate Note: MW bands detected vary by cell line or sample with detection of zymogen and/or active forms. Immunoprecipitation: confirm by immunoblot using clone 5D4E11B6; 1:50 using clone 5C6D5 (Catalog # MAB1771) to immunoprecipitate ADAMTS-1 from 500 µg of HEK293 whole cell lysate or PC-12 whole cell lysate |
| Biological Information | |
|---|---|
| Immunogen | Myc-His carboxyl tagged full-length recombinant protein based on the human sequence. |
| Epitope | Unknown |
| Clone | 5D4E11B6 |
| Host | Mouse |
| Specificity | Clone 5D4E11B6 recognizes human ADAMTS-1. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Reactivity with other species has not been tested. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. CATALYTIC ACTIVITY: Cleaves aggrecan at the 1938-Glu-|-Leu-1939 site, within the chondroitin sulfate attachment domain. COFACTOR: Binds 1 zinc ion per subunit (By similarity). SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity). DOMAIN: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix. DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. PTM: The precursor is cleaved by a furin endopeptidase (By similarity). SIMILARITY: Contains 1 disintegrin domain and contains 1 peptidase M12B domain and contains 3 TSP type-1 domains. |
| Molecular Weight | 110 / 87 / 65 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C for up to 1 year from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |