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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, FC, FUNC, ICC, IHC | AHm | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1397Z |
| Replaces | CBL1331F |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-ICAM-1 Antibody, clone 3E2B, Azide Free |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified IgG prepared by affinity chromatography on Protein G agarose beads. Liquid at 1 mg/mL in sterile-filtered PBS, containing no preservatives. Endotoxin level 4.13EU/mg |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-ICAM-1 Antibody, clone 3E2B, Azide Free detects level of ICAM-1 & has been published & validated for use in ELISA, FC, FUNC, IC, IH. |
| Key Applications |
|
| Application Notes | Immunocytochemistry: Live or acetone fixed cells ELISA Immunohistochemistry of acetone fixed frozen sections. (Paraffin not tested). Flow Cytometry: Suggested working dilution 50 μg/mL. Use 5 mL of this dilution to label 0.5 x 10(e6) cells. Immunoprecipitation: use protein G or rabbit anti-hamster IgG secondary Clone 3E2B is reported to be function blocking against murine ICAM-1. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Clone | 3E2B |
| Host | Armenian Hamster |
| Specificity | MAB1371Z is reactive with mouse ICAM-1 (CD54), which is present on T-cells, B-cells, monocytes and endothelial cells, epithelial cells, dendritic cells and keratinocytes. Expression of CD54 is increased after stimulation with cytokines and LPS, and it also plays a role in B cell-T cell interactions. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | ICAM1 (CD54) is typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. ICAM1 is also exploited by Rhinovirus as a receptor. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P05362 # ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). In case of rhinovirus infection acts as a cellular receptor for the virus. Also acts as receptor for some coxsackieviruses. SIZE: 532 amino acids; 57825 Da SUBUNIT: Interacts with human herpesvirus 8 MIR2 protein (Probable). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. PTM: Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. SIMILARITY: SwissProt: P05362 ## Belongs to the immunoglobulin superfamily. ICAM family. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |