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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, R, Rb | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB13170 |
| Description | Anti-Cav1.2 calcium channel Antibody, clone L57/46 |
| Alternate Names |
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| Background Information | This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Many alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Cav1.2 calcium channel Antibody, clone L57/46 is validated for use in WB, IH for the detection of Cav1.2 calcium channel. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect Cav1.2 calium channel in human kidney tissue. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to rabbit Cav1.2 calcium channel at the cytoplasmic domain proximal to the C-terminus. |
| Epitope | Cytoplasmic domain |
| Clone | L57/46 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes Cav1.2 calcium channel at the cytoplasmic domain. |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | Demonstrated to react with mouse and human. Predicted to react with rat and rabbit based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the high-voltage activated (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function (By similarity). SUBUNIT STRUCTURE: Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with CABP1 and CACNA2D4 By similarity. Interacts (via C-terminal CDB motif) with CABP5; in a calcium-dependent manner (By similarity). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Cell membrane. Note: The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane. TISSUE SPECIFICTY: Expression in cardiac muscle. In lung, expressed in airway and vascular smooth muscle cells. DOMAIN: Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel. PTM: Phosphorylation by PKA activates the channel. SEQUENCE SIMILARITIES: Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. (View classification). |
| Molecular Weight | Observed at ~ 240 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse brain tissue lysate. Western Blot Analysis: 2 µg/mL of this antibody detected Cav1.2 calcium channel on 10 µg of mouse brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |