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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P), IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1120 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified from ascites fluid by Protein G chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31 detects level of Insulin-like Growth Factor-1 Receptor & has been published & validated for use in IH(P) & IP. |
| Key Applications |
|
| Application Notes | Acts as a Weak IGF-like Agonist in Bioassays (Thymidine incorporation into DNA) Immunoprecipitation (Use Protein G): 2μg antibody/mg protein lysate. Immunohistochemistry (Frozen and formalin/paraffin): Use antibody at 2-4ug/mL for 30 min. at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min. followed by cooling at room temperature for 20 minutes. Please note that EDTA is better than citrate for epitope unmasking. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA. |
| Clone | 24-31 |
| Host | Mouse |
| Specificity | Recognizes the alpha-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 283-440 (exon 4-6) of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The a-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. It is assumed that, as for the insulin receptor, the tyrosine kinase activity is essential for IGF-1 action. Weakly reacts with Rabbit. |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P08069 # This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. SIZE: 1367 amino acids; 154793 Da SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed in a variety of tissues. PTM: The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin- like growth factor I (IGF I). & Phosphorylation of Tyr-980 is required for IRS1- and SHC1- binding. DISEASE: SwissProt: P08069 # Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1. SIMILARITY: SwissProt: P08069 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 3 fibronectin type-III domains. & Contains 1 protein kinase domain. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |