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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ELISA, ICC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1049 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Bone Morphogenetic Protein 4 Antibody, clone 3H2 |
| Alternate Names |
|
| Background Information | Bone morphogenetic protein 4 (BMP4) is a polypeptide belonging to the TGF-β superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signalling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. If these cells also receive signals from FGF, they will differentiate into the spinal cord; in the absence of FGF the cells become brain tissue. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Bone Morphogenetic Protein 4 using this Anti-Bone Morphogenetic Protein 4 Antibody, clone 3H2 validated for use in ELISA, IC & WB. |
| Key Applications |
|
| Application Notes | Immunocytochemistry using osteoinductive cells; i.e., BFP and Saos-2 Western blot using osteoinductive cells; i.e., BFP and Saos-2 Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant mouse BMP-4 (Masuhara, 1995). |
| Clone | 3H2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Specific for bone morphogenetic protein-4 (BMP-4) by Western blot and ELISA. No cross-reactivity with human BMP-2 or human TGF-β1. The antibody reacts with both human and mouse BMP-4 in both reduced and nonreduced conditions. Human BMP-4 (accession number P12644) precursor (389 aa, glycosilated) consists of a propeptide (273 aa) and active BMP-4 (116 aa, glycoslyated; MW reduced 17-20 kDa). |
| Isotype | IgG2b |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5 untranslated region of this gene has been described and three variants are described, all encoding an identical protein. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P12644 # Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. SIZE: 408 amino acids; 46555 Da SUBUNIT: Homodimer; disulfide-linked (By similarity). Interacts with GREM2 (By similarity) and SOSTDC1. Part of a complex consisting of TWSG1 and CHRD (By similarity). SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. TISSUE SPECIFICITY: Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. SIMILARITY: SwissProt: P12644 ## Belongs to the TGF-beta family. |
| Stem Cell Type |
|
| Molecular Weight | 17 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |