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Millipore Engineered Bfgf Growth Factor With Enchanced Thermostability Compared To The Wild-Type Bfgf Protein. Allows A Less Vigorous Feeding Schedule For Human Es/Ips And Neural Stem Cell Culture. - Mill (Additional S&H or Hazmat Fees May Apply)
HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.
Product is endotoxin free (<0.5 ng/μg of protein (<5 EU/μg) and greater than 95% pure by SDS-PAGE.
Description
Catalogue Number
GF446-10UG
Trade Name
HumanKine®
Description
HumanKine® Thermostable bFGF, Human Recombinant
Alternate Names
FGF2
prostatropin
Prostatropin
HBGF-2
BFGF
Background Information
The HumanKine Thermostable bFGF is engineered with enchanced thermostability compared to the wild-type bFGF protein without modification of biological function.
Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 154 amino acid residues. bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions.
Product Information
Presentation
Lyophilized from a 0.2 μm filtered solution of 1X PBS.
Quality Level
MQ100
Applications
Application
Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.
Application Notes
HumanKine products are Xeno-Free
Biological Information
Purity
Product is endotoxin free (<0.5 ng/μg of protein (<5 EU/μg) and greater than 95% pure by SDS-PAGE.
Source
HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.
UniProt Number
P09038
Product Usage Statements
Quality Assurance
Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of the 3T3 cell line. EC50 is typically ≤0.5 ng/mL.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -20°C for up to 4 months from date of receipt. Further dilutions should be made into appropriate buffer or medium to which protein has been added.
