Key Specifications Table
| Source | Purity |
|---|---|
| Protein is expressed in E.coli. The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided. | Product endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Description | |
|---|---|
| Catalogue Number | GF348 |
| Description | TWEAK, Human Recombinant Animal Free |
| Alternate Names |
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| Background Information | TWEAK belongs to the TNF family of ligands and signals through TWEAKR also known as TNFRSF12A. TWEAK is expressed in a variety of tissues, including the adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK has the ability to induce NFkB activation and chemokine secretion, and to exert an apoptotic activity in certain cells, such as HT-29 human adenocarcinoma cells when cultured in the presence of IFN-. TWEAK also promotes proliferation and migration of endothelial cells. Recombinant human TWEAK is a soluble 17.0 kDa polypeptide (154 amino acid residues) comprising the TNF homologous region of TWEAK and is generated by proteolytic processing of the full length membrane anchored TWEAK protein. |
| Product Information | |
|---|---|
| Presentation | Product is presented in 10 mM Sodium Phosphate, pH 7.5 + 80 mM L-Arginine and is filtered through a 0.2 micron filter before lyophilization. |
| Quality Level | MQ100 |
| Biological Information | |
|---|---|
| Purity | Product endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Source | Protein is expressed in E.coli. The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided. |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Assay #1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml. Assay #2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-γ. The ED50 for this effect is between 30-45 ng/ml. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 25µg |