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Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
Description
Catalogue Number
GF316
Description
EGF Protein, Human Recombinant Animal Free
Alternate Names
Epidermal Growth Factor
Urogastrone
Background Information
Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGFα, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparinbinding EGF like growth factor (HB-EGF), epigen, and the neuregulins (NRG)1-6. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). Recombinant human EGF is a 6.2 kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds.
Product Information
Formulation
Presentation
Product is filtered through a 0.2 micron filter before lyophilization.
Quality Level
MQ100
Applications
Application
This Recombinant animal free human EGF is manufactured using all non-animal reagents.
Biological Information
Purity
Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
Source
E. coli
Cross Reactivity
Cow, Hamster, Monkey, Mouse, Pig, Rabbit, Rat
Entrez Gene Number
NM_001963.2
UniProt Number
P01133
Product Usage Statements
Quality Assurance
Bioactivity assay: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤0.1 ng/mL, corresponding to a specific activity of ≥1 x 107 units/mg.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -20°C for up to 6 months from date of receipt.
