Key Specifications Table
| Key Applications | Species | Source | Purity |
|---|---|---|---|
| CULT, Cult | Human | E.coli | Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1 EU/ug) of BAFF. |
| Description | |
|---|---|
| Catalogue Number | GF136 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | BAFF Protein, Recombinant human |
| Overview | Human BAFF (B-cell activating factor belonging to the TNF family) is a novel ligand in the TNF family. BAFF plays an important role as co-stimulator of B-cell proliferation and function. Recombinant human BAFF is a soluble 17.0 kDa protein containing 153 amino acid residues, comprising the TNF-like extracellular domain of BAFF. |
| Alternate Names |
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| Product Information | |
|---|---|
| Presentation | Lyophilized from sterile filtered 0.5X PBS, pH 7.5, and 0.5 mM DTT, containing no additives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | The Human BAFF protein (B-cell activating factor belonging to the TNF family) is a novel ligand in the TNF family. |
| Key Applications |
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| Biological Information | |
|---|---|
| Purity | Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1 EU/ug) of BAFF. |
| Source | E.coli |
| Specific Activity | Human BAFF is biologically active when compared to standards. The ED50 as determined by its dose-dependent mitogenic activity on human RPMI 8226 cells is 5-10 ng/mL. |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q9Y275 # Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. SIZE: 285 amino acids; 31223 Da SUBUNIT: Homotrimer. SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor ligand superfamily member 13b, soluble form: Secreted. TISSUE SPECIFICITY: Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T- cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. PTM: The soluble form derives from the membrane form by proteolytic processing. & N-glycosylated. SIMILARITY: SwissProt: Q9Y275 ## Belongs to the tumor necrosis factor family. |
| Stem Cell Type |
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| Molecular Weight | 17.0 kDa |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | The lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C for up to one year from date of receipt. Reconstituted material can be stored at 2°-8°C for one week, but undiluted aliquots at -20°C are recommended. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 20 µg |