Key Specifications Table
| Key Applications | Species | Source | Purity |
|---|---|---|---|
| CULT | Rat | E.coli | Greater than 98% by SDS-PAGE analysis and HPLC analysis. |
| Description | |
|---|---|
| Catalogue Number | GF041 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Monocyte Chemotactic Protein-1, Recombinant rat |
| Overview | Macrophage/monocyte chemoattractant protein-1 (MCP-1) plays an important role in the inflammatory response of blood monocytes and tissue macrophages. Rat MCP-1 is a 14.1 kDa protein containing 125 amino acid residues. |
| Alternate Names |
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| Product Information | |
|---|---|
| Presentation | Lyophilized protein with no additives. The protein may appear as a haze or film which is difficult to see at the bottom of the vial. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | The Macrophage/monocyte chemoattractant protein-1 (or MCP-1 protein) plays an important role in the inflammatory response of blood monocytes & tissue macrophages. |
| Key Applications |
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| Biological Information | |
|---|---|
| Purity | Greater than 98% by SDS-PAGE analysis and HPLC analysis. |
| Source | E.coli |
| Specific Activity | Determined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P13500 # Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. SIZE: 99 amino acids; 11025 Da SUBUNIT: Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. SUBCELLULAR LOCATION: Secreted. PTM: Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. SIMILARITY: SwissProt: P13500 ## Belongs to the intercrine beta (chemokine CC) family. |
| Stem Cell Type |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | The lyophilized powder, though stable at room temperature, is best stored desiccated at -20°C. After a quick spin, reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 4°C for one week or -20°C for future use. After reconstitution store in working aliquots at -20°C for up to 6 months. |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |