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Millipore Detect Tra-2-49 Using This Milli-Mark Anti-Tra-2-49 Antibody, Clone Tra-2-49/6e, Fitc Conjugate Validated For Use In Fc. - Mill (Additional S&H or Hazmat Fees May Apply)
In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification. Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.
Product Information
Format
FITC
Control
2102 Ep cells.
Presentation
Purified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality Level
MQ100
Applications
Application
Detect TRA-2-49 using this Milli-Mark Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate validated for use in FC.
Key Applications
Flow Cytometry
Working Solution
Cells / test
Total Reaction Volume
5 μl
5 x 105 Cells / 95 μl PBS
100 μl
10 μl
1 x 106 Cells / 90 μl PBS
100 μl
Biological Information
Immunogen
2102Ep human Embryonal Carcinoma Cells
Epitope
Unknown
Clone
TRA-2-49/6E
Host
Mouse
Specificity
Antibody recognizes TRA-2-49.
Isotype
IgG1
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
TRA-2-49
Purification Method
Protein A Purfied
Molecular Weight
212/80/55 kDa Observed
Product Usage Statements
Quality Assurance
Evaluated by flow cytometry using 2102 Ep cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
