| Description | |
|---|---|
| Catalogue Number | CC1021 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Human Integrin αVβ3, Octyl-β-D-glucopyranoside Formulation |
| Overview | Integrin alphaVbeta3 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to alphaVbeta3 integrin. A tissue detergent extract, which was applied to the column was prepared as previously described [Belkin, 1990]. Product was tested and found negative for HIV and Hepatitis B. |
| Alternate Names |
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| Product Information | |
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| Presentation | Purified protein inTris buffered saline with 100 mM Octyl-beta-D-glucopyranoside, and sodium azide. This format of alphaVbeta3 is appropriate for functional evaluations. |
| Quality Level | MQ100 |
| Applications | |
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| Key Applications |
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| Application Notes | Electrophoresis and Immunoblotting control. Also useful for in vitro binding experiments with metalloproteinase MMP-2 . |
| Biological Information | |
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| Concentration | 0.351 mg/mL |
| Purity | >95% by SDS-PAGE (silver stain) |
| Specific Activity | FUNCTIONAL ACTIVITY: <br /><br />Confirmed by vWf (von Willibrand factor) binding assay. It is expected that this preparation of alphaVbeta3 integrin will also interact with other alphaVbeta3 binding proteins in an RGD-dependent fashion. |
| Entrez Gene Number |
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| Entrez Gene Summary | ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; ITGB1, ITGB3, ITGB5, ITGB6, and ITGB8), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposis sarcoma lesions. SIZE: 1048 amino acids; 116038 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain at -70°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/ thaw cycles. |
| Packaging Information | |
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| Material Size | 50 µg |