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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IP, WB, IHC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL553 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-CD63 Antibody, clone RFAC4 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD63 Antibody, clone RFAC4 is an antibody against CD63 for use in FC, IP, WB, IH, IH(P). |
| Key Applications |
|
| Application Notes | Flow cytometric studies of CD63 expression Immunoprecipitation Western blotting of CD63 antigen from solubilized platelet proteins Suitable for formalin fixed paraffin wax embedded tissue sections. HIER and proteolytic pretreatment may not be necessary. Users should confirm specificity and pretreatment for their particular application. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Clone | RFAC4 |
| Host | Mouse |
| Specificity | This antibody reacts with a 53 kDa protein which form a part of the family of tetraspan moieties. The antigen was originally designated as a lysosomal membrane protein characterized as an activation dependent platelet surface antigen. In fact the CD63 antigen has a diverse distribution on the surface and in the cytoplasm of many cell types including lymphoid, myeloid, endothelial cells and melanoma. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene. Alternative splicing results in multiple transcript variants encoding different proteins. |
| Gene Symbol |
|
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P08962 # This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. SIZE: 238 amino acids; 25637 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note=Also found in Weibel- Palade bodies of endothelial cells. TISSUE SPECIFICITY: Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. SIMILARITY: SwissProt: P08962 ## Belongs to the tetraspanin (TM4SF) family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |