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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, FUNC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL517 |
| Replaces | CBL517P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-CD28 Antibody, clone 15E8 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Please see lot specific datasheet for product formulation. We recommend that each laboratory determine an optimum working titre for use in its particular application. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-CD28 Antibody, clone 15E8 is validated for use in FC, FUNC for the detection of CD28. |
| Key Applications |
|
| Application Notes | To study the role of CD28 in T cell proliferation. The antibody will induce the proliferation of resting T lymphocytes. This antibody will act in cell stimulation studies where it has been suggested that measuring T cell function in HIV infected asymptomatic patients may be an early indicator of AIDS, independent of CD4 + cell counts Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Human T lymphocytes |
| Clone | 15E8 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody reacts with the 44 kDa antigen found on a sub-population of T cells and activated B cells. These CD28+ CD8 cells are now known to be cytotoxic T cell precursors. The antibody does not react with B cells, granulocytes or monocytes. The ligand for CD28 is CD80 (B7) which is found on activated B cells suggesting a role for CD28 in T and B cell interaction FUSION PARTNER: SP2/0 |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | CD28 costimulation is essential for CD4 (MIM 186940)-positive T-cell proliferation, survival, interleukin-2 (IL2; MIM 147680) production, and T-helper type-2 (Th2) development.[supplied by OMIM] |
| Gene Symbol |
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| Non-Reactive Species |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P10747 # Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. SIZE: 220 amino acids; 25066 Da SUBUNIT: Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed in T-cells and plasma cells, but not in less mature B-cells. SIMILARITY: SwissProt: P10747 ## Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |