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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Av, Ch, H, Ma, Po, R, Rp | IHC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL212 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Neurofilament 200 kDa Antibody, clone RT97 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | The monoclonal is presented in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Neurofilament 200 kDa Antibody, clone RT97 detects level of Neurofilament 200 kDa & has been published & validated for use in IH, IH(P). |
| Key Applications |
|
| Application Notes | Staining of both frozen and paraffin wax embedded tissue sections Strong staining of neuronal processes in a variety of tissues Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Clone | RT97 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | The clone RT97 recognizes the 200 kDa neurofilament polypeptide in a number of mammalian and avian species. Tumours specifically recognized include phaeochromocytoma, paraganglioma, ganglioneuroblastoma and other tumours of neuronal origin. KNOWN SPECIES CROSS REACTIVITY: Recognizes rat, chicken, pig and reptile |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. SIZE: 1026 amino acids; 112480 Da PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function. DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |