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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, FC, IHC, NEUT | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL2117 |
| Replaces | 04-1095 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Interleukin-6 Antibody, clone B-E8 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | The monoclonal is purified from tissue culture supernatant by ion exchange chromatography and is presented as a liquid in phosphate buffered saline, pH 7.2. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Interleukin-6 using this Anti-Interleukin-6 Antibody, clone B-E8 validated for use in ELISA, FC, IH, NEUT. |
| Key Applications |
|
| Application Notes | Blocks Interleukin-6 induced proliferation on B9 cells: 20pg of antibody neutralizes the activity of 1U of IL-6. It is recommended that the sodium azide is removed from the antibody prior to use in this application. Immunohistochemistry on frozen sections Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human IL-6. |
| Clone | B-E8 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes natural and recombinant forms of human Interleukin-6 with an affinity of 0.5 nM. Inhibits IL-6 binding to its receptors and blocks Interleukin-6 induced proliferation on B9 cells. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Non-Reactive Species |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P05231 # IL-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants. SIZE: 212 amino acids; 23718 Da SUBCELLULAR LOCATION: Secreted. PTM: N- and O-glycosylated. DISEASE: SwissProt: P05231 # Genetic variations in IL6 are associated with susceptibility to systemic juvenile rheumatoid arthritis [MIM:604302]. Systemic juvenile rheumatoid arthritis is juvenile chronic arthritis associated with severe, debilitating, extraarticular features, and occasionally fatal complications. Despite medical treatment, many children still experience early joint destruction, necessitating surgical replacement. & At least 1 polymorphism in the IL6 gene renders HIV- infected men susceptible to Kaposi sarcoma [MIM:148000]. Kaposi sarcoma is a sarcoma of spindle cells mixed with angiomatous tissue. A relatively rare malignant skin tumor that results in multifocal purplish coloured papules or plaques that eventually form nodules. Most commonly seen in patients who suffer from AIDS. SIMILARITY: SwissProt: P05231 ## Belongs to the IL-6 superfamily. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at 2-8°C for up to 12 months from date of receipt. For long term storage aliquot antibody into small volumes and store at -20°C. Avoid repeated freeze-thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |