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Millipore Detect Kinectin Using This Anti-Kinectin Antibody Validated For Use In Western Blotting, Icc. - Mill (Additional S&H or Hazmat Fees May Apply)
Organelle transport is facilitated by molecular motors, such as kinesin and dynein, which interact with microtubules to transport cargo in an ATP-dependent manner. The shuttling of organelles is also dependent on kinectin, a transmembrane ER protein that associates with kinesin and stimulates its ATPase activity. Kinesin-kinectin dynamics have also been linked to focal adhesion growth. In addition, kinectin has been shown to activate the RhoG GTPase pathway which involves Rac1 and Cdc42 signaling. Kinectin is expressed in testis, ovary, spleen, thymus, prostate, colon, and small intestine.
Product Information
Format
Affinity Purified
Control
Human testis tissue lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Kinectin using this Anti-Kinectin Antibody validated for use in Western Blotting, ICC.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Kinectin in HeLa and A431 cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the lumenal region of human Kinectin.
Epitope
Lumenal region
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes the lumenal region of Kinectin.
Species Reactivity
Human
Species Reactivity Note
Demonstrated to react with Human. Predicted to react with Rhesus Macaque based on 100% sequence homology. Other homologies: Mouse (82% Mouse sequence homology) and Rat (79% sequence homology).
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001072989
Gene Symbol
KTN1
CG1
Purification Method
Affinity Purfied
UniProt Number
Q86UP2
Molecular Weight
~158 kDa observed. Uniprot describes 3 isoforms at ~149, ~150, and ~156 kDa.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in human testis tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Kinectin in 10 µg of human testis tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
