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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Mk | ICC, WB, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT80 |
| Description | Anti-Girdin, coiled-coil region Antibody |
| Background Information | Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Girdin, coiled-coil region Antibody is validated for use in ICC, Western Blotting, Immunoprecipitation for the detection of Girdin, coiled-coil region. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected Girdin in Cos-7 cells (Prof. P. Ghosh, Department of Medicine, Division of Gastroenterology and Hepatology, UCSD School of Medicine and Veterans Affairs Medical Center). Western Blot Analysis: A representative lot detected Girdin in Cos-7 cell lysate. Uncharacterized bands may be observed in some cell lysates. Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in HeLa cell lysates (Prof. P. Ghosh, Department of Medicine, Division of Gastroenterology and Hepatology, UCSD School of Medicine and Veterans Affairs Medical Center). Uncharacterized bands may be observed in some cell lysates. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to the coiled coil domain of rat Girdin. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human and Monkey. Predicted to react with Rat. Other Homologies: Mouse (97% sequence homology). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Purification Method | Affinity Purfied |
| Molecular Weight | ~250 kDa observed. Some uncharacterized bands may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Immunocytochemistry Analysis: A 1:1000 dilution of this antibody detected Girdin in HeLa cells (Prof. A. Ghosh, Division of Biology, UCSD). |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |