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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT47 |
| Description | Anti-Claudin-7 Antibody |
| Alternate Names |
|
| Background Information | Claudin-7 is a member of a family of claudins that are tight junction integral membrane proteins with four transmembrane domains and two extracellular loops. Claudin-7 has been shown to be under-expressed in invasive ductal carcinomas and colorectal carcinomas as compared to normal epithelial cells. Claudin-7 differs from other claudins because it has both structural and regulatory functions, regulating the expression of prostate specific antigen. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Claudin-7 Antibody is an antibody against Claudin-7 for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Claudin-7. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc. Alternatively spliced transcript variants encoding different isoforms have been found. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Plays a major role in tight junction-specific obliteration of the intercellular space (By similarity). SUBUNIT STRUCTURE: Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (By similarity). The phosphorylated form interacts with EPCAM. Ref.10 SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Lateral cell membrane. Cell junction › tight junction. Note: Co-localizes with EPCAM at the lateral cell membrane and tight junction. TISSUE SPECIFICTY: Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions. INDUCTION: By androgens. PTM: Phosphorylated. SEQUENCE SIMILARITIES: Belongs to the claudin family. SEQUENCE CAUTION: The sequence AAP97219.1 differs from that shown. Reason: Frameshift at positions 18 and 23. |
| Molecular Weight | ~22 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in BALB3T3/A31 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected Claudin-7 in 10 µg of BALB3T3/A31 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |