Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT273 |
| Description | Anti-SUN1 Antibody |
| Alternate Names |
|
| Background Information | SUN domain-containing protein 1 (SUN1), also known as Protein unc-84 homolog A or Sad1/unc-84 protein like 1 (UNC84A), is a component of the SUN-protein-containing complexes also called LINC (linker of nucleoskeleton and cytoskeleton) complexes. These complexes link the nucleoskeleton and cytoskeleton structures together in a scaffold like fashion. SUN1 is required for interkinetic nuclear migration and nucleokinesis as well as nucleus-centrosome coupling during radial neuronal migration and glial migration in the cortex. SUN1 also is important during meiosis and is required for proper gametogenesis as well. Additionally, SUN1 it is important in the distribution of nuclear pore complexes on the nuclear membrane. Interestingly, LINC complexes are also critical for vital functions such as hearing. For instance, recent research demonstrates that the position of the nucleus in sensory epithelial cells, partly governed by SUN1, appears critical for normal hearing. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect SUN1 using this Anti-SUN1 antibody validated for use in western blotting & ICC. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:1;000 dilution from a representative lot detected SUN1 in 10 µg of HepG2, Jurkat, and PC3 cell lysate. Western Blotting Analysis: A representative lot detected SUN1 recombinant protein (Haque, F., et al. (2010). Journal of Biol. Chem. 285(5): 3487-3498). Immunocytochemistry Analysis: A representative lot detected SUN1 in human foreskin fibroblasts cells (Haque, F., et al. (2010). Journal of Biol. Chem. 285(5): 3487-3498). |
| Biological Information | |
|---|---|
| Immunogen | MBP-tagged recombinant protein corresponding to the perinuclear space domain of human SUN1. |
| Epitope | Perinuclear space domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~ 110 kDa observed. The calculated molecular weight is 90 kDa, however Sun1 has been shown as a ~80-110 kDa band in western blots (Gob, E., et al. (2011). Commun. Integr Biol. 4(4):440-442). Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: A 1:1;000 dilution of this antibody detected SUN1 in 10 µg of A431 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |