Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT242 |
| Description | Anti-Galectin-4 Antibody |
| Alternate Names |
|
| Background Information | Galectin 4, or GAL-4, is another member of the Galectin family. Galectins are carbohydrate binding proteins that appear important in cell-cell contact, cell migration, and cell to cell signaling in both normal and disease (cancerous) tissues. Galectin 4 differs from its sister family members in the range of carbohydrate substrates that it binds. Galentin-4 is synthesized as a cytosolic protein, but can be externalized. During development and in adult normal tissues, galectin-4 is expressed only in the alimentary tract, from the tongue to the large intestine. It is often found in relatively insoluble complexes as a component of either adherent junctions or lipid rafts in the microvillus membrane, and it has been proposed to stabilize these structures. Strong expression of galectin-4 can be induced, however, in cancers from other tissues including breast and liver. Also, Galectin-4 appears to specifically bind CD3 on activated T-cells in the gut and regulate their activity via calpain mediated apoptosis. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Galectin-4 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Galectin-4. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Galectin-4 in 10 µg of Colo320 cell lysate. Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Galectin-4 in 10 µg of human small intestine tissue lysate. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Galectin-4 in human stomach tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Galectin-4 near the N-terminal. |
| Epitope | near N-terminal |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Monkey, Chimpanzee, Primate, Rhesus Macaque, Feline based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~36 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HT-29 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Galectin-4 in 10 µg of HT-29 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |