| Description | |
|---|---|
| Catalogue Number | ABT1838 |
| Description | Anti-Claudin-16 |
| Alternate Names |
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| Background Information | Claudin-16 (UniProt: Q9Y5I7; also known as Paracellin-1, PCLN-1) is encoded by the CDLN1 (also known as PCLN1) gene (Gene ID: 10686) in human. Claudin-16 is a multi-pass membrane protein that span the plasma membrane 4 times, with their N- and C-termini located in the cytosol. It is selectively expressed at tight junctions of renal epithelial cells of the thick ascending limb of loop of Henle s where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of divalent cations. Claudin-16 may form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors. Over 20 different mutation sin CLDN1 gene have been reported that are linked to familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC), a renal Mg2+ and Ca2+ wasting disorder that ultimately leads to progressive kidney failure. (Ref.: Muller D et al. (2006). J. Clin. Endocrinol. Metab. 91(8):3076-3079). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.54 M Tris, 0.2 M Glycine, 250 mM NaCl, pH 7.5 without azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rabbit polyclonal Anti-Claudin-16, Cat. No. ABT1838, is a rabbit polyclonal antibody that detects Claudin-16 and has been tested for use in Immunocytochemistry, Immunofluorescence, and Inhibition/Function studies. |
| Key Applications |
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| Application Notes | Immunofluorescence Analysis: A representative lot detected Claudin-16 in Immunofluorescence applications (Kausalya, P.J., et. al. (2006). J Clin Invest. 116(4):878-91). Inhibits Activity/Function: A representative lot blocked Clathrin-mediated endocytosis in MDCK cells (Muller, D., et. al. (2006). J Clin Endocrinol Metab. 91(8):3076-9). |
| Biological Information | |
|---|---|
| Immunogen | Conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Claudin-16. |
| Epitope | extracellular domain |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Claudin-16 in human and canine cells. It targets an epitope within 15 amino acids from the N-terminal region. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Canine, Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | 33.84 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in MDCK cells. Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected Claudin-16 in Madin-Darby Canine Kidney Epithelial (MDCK) cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |