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Millipore Detect Protocadherin Using This Rabbit Polyclonal Antibody, Anti-Protocadherin-10 Antibody Validated For Use In Western Blotting & Ihc (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
Protocadherin-10 is a Single-pass type I membrane protein that is likely involved in calcium-dependent cell-adhesion. Protocadherin-10 is moderately expressed in brain testis and ovaries, with low expression in most other tissues.
Product Information
Format
Affinity Purified
Control
HEK293 cell lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Protocadherin using this rabbit polyclonal antibody, Anti-Protocadherin-10 Antibody validated for use in western blotting & IHC (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Protocadherin-10 in human cerebellum, thalamus, and testis tissues.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the Cadherin 6 domain of human Protocadherin-10.
Epitope
Cadherin 6 domain
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes the Cadherin 6 domain of Protocadherin-10.
Species Reactivity
Human
Species Reactivity Note
Demonstrated to react with Human. Predicted to react with Mouse, Bovine based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_116586
Gene Symbol
PCDH10
Purification Method
Affinity Purfied
UniProt Number
Q9P2E7
Molecular Weight
~130 kDa observed. The calculated molecular weight of this protein is 113 kDa. However, Uniprot states that this protein is glycosylated, which may result in higher molecular weights. Uncharacterized band(s) may be observed in some cell lysates.
Product Usage Statements
Quality Assurance
Evlauted by Western Blotting in HEK293 cell lysates.
Western Blotting Analysis: 2 µg/mL of this antibody detected Protocadherin-10 in 10 µg of HEK293 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
