The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Millipore This Anti-Rab8a Antibody Is Validated For Use In Western Blotting For The Detection Of Rab8a. - Mill (Additional S&H or Hazmat Fees May Apply)
Ras-related protein Rab-8A (RAB8A) is one of two Rab8 isoforms, which belong to the superfamily of GTPases. They cycle between an active GTP-bound state, formed from interaction with guanine nucleotide exchange factors (GEFs) such as Rabin8 and GRAB; and an inactive GDP-bound state via interaction with GTPase activating proteins (GAPs) such as TBC1D1. Rab8A activates various effector molecules, which are coupled to intracellular signaling networks that mediate membrane and vesicular trafficking, and actin remodeling. Rab8A is found in the golgi network, perinuclear region, plasma membrane, lamellipodia, and cilia, among other regions.
Product Information
Format
Affinity Purified
Control
Serum starved HeLa cell lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-RAB8A Antibody is validated for use in Western Blotting for the detection of RAB8A.
Key Applications
Western Blotting
Application Notes
Western Blot Analysis: A representative lot from an independent laboratory detected RAB8A in human cerebellum and brain.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of human RAB8A.
Epitope
C-terminus
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes the C-terminus of RAB8A.
Species Reactivity
Human
Species Reactivity Note
Demonstrated to react with Human. Predicted to react with Mouse and Bovine based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_005361
Gene Symbol
RAB8A
MEL
RAB8
Purification Method
Affinity Purfied
UniProt Number
P61006
Molecular Weight
~24 kDa observed. Uncharacterized bands may be observed at ~110 kDa and ~38 kDa.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in serum starved HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected RAB8A in serum starved HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
