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Millipore Detect Collagen Type I Using This Anti-Collagen Type I Antibody Validated For Use In Western Blotting, Ihc(P), Elisa. - Mill (Additional S&H or Hazmat Fees May Apply)
Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.
Product Information
Format
Affinity Purified
Control
Rat skin tissue
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Collagen Type I using this Anti-Collagen Type I Antibody validated for use in Western Blotting, IHC(P), ELISA.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
ELISA
Application Notes
Specificity ELISA Analysis: A representative lot detected mouse and rat collagen but not human collagen in ELISA.
Western Blot Analysis: A 1:2,000 dilution from a representative lot detected Collagen Type 1 on 10 µg of rat skin tissue lysate.
Biological Information
Immunogen
Rat tail collagen
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Mouse
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_445756
Gene Symbol
COL1A1
Purification Method
Affinity Purfied
UniProt Number
P02452
Molecular Weight
130 kDa calculated
Product Usage Statements
Quality Assurance
Evaluated by Immunohistochemistry in rat skin tissue.
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Collagen Type 1 in rat skin tissue.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
