Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, B, Eq, M | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT121 |
| Description | Anti-T-cadherin (CDH13) Antibody |
| Alternate Names |
|
| Background Information | T-cadherin (CDH13), also known as Cadherin 13, is a member of the cadherin superfamily. This protein is unique from its other family members in that it lacks the cytoplasmic domains and is anchored to the cells membrane through the GPI anchor. T-cadherin is widely expressed in the cardiovascular system. Mutations in T-cadherin have been associated with many types of cancer. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-T-cadherin (CDH13) Antibody detects level of T-cadherin (CDH13) & has been published & validated for use in WB, IH(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected T-cadherin in human brain and human heart tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human T-cadherin at the Cadherin 1 domain. |
| Epitope | Cadherin 1 domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes T-cadherin at the Cadherin 1 domain. This antibody detects the ~130 kDa pro-protein and the proteolytically cleaved ~105 kDa mature isoforms. (Denzel, M., et al. (2010). J Clin Invest.120(12):4342–4352.) |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human and Rat. Predicted to react with Bovine, Equine, and Mouse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the cadherin superfamily. The encoded protein is localized to the surface of the cell membrane and is anchored by a GPI moiety, rather than by a transmembrane domain. The protein lacks the cytoplasmic domain characteristic of other cadherins, and so is not thought to be a cell-cell adhesion glycoprotein. This protein acts as a negative regulator of axon growth during neural differentiation. It also protects vascular endothelial cells from apoptosis due to oxidative stress, and is associated with resistance to atherosclerosis. The gene is hypermethylated in many types of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2011]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor › GPI-anchor TISSUE SPECIFICITY: Highly expressed in heart. In the CNS, expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. No expression detected in cerebellum or spinal cord. DEVELOPMENTAL STAGE: Expressed at higher levels in adult brain than in developing brain. SEQUENCE SIMILARITIES: Contains 5 cadherin domains. |
| Molecular Weight | ~130 and ~105 kDa observed. ~130 kDa corresponds to the pro-protein and ~105 kDa corresponds to the proteolytically cleaved mature isoform. (Denzel, M., et al. (2010). J Clin Invest.120(12):4342–4352.) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human heart tissue lysate. Western Blot Analysis: 0.1 µg/mL of this antibody detected T-cadherin on 10 µg of human heart tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |